Description
产品说明
| 所属分类 | 微生物 > 模式菌株 |
|---|---|
| 品牌 / 来源 | CS-BIC | 中科生信 |
| 保存温度 | 冷藏4-10℃ |
| 参考用途 | 模式菌株 |
| 培养温度 | 30℃ |
| 提供方式 | 培养物 |
| 来源历史 | 咨询客服 |
| 模式菌株 | 模式菌株 |
| 生物危害程度 | 四类 |
| 其它保藏中心编号 | =KCTC 4672 |
| 培养条件 | 1055sodium acetate0.82gCaCl2·2H2O0.147gNH4Cl0.535g NaH2PO40.136gNa2SO40.142gTrace element solution10.0mlVitamins solution（见培养基0901）10.0ml Se/W solution（见培养基0975）1.0mlDistilled water 1.0L Mix components thoroughly and autoclave under a N2-CO2 (80:20 v/v) gas mixture. After cooling add aseptically and anaerobically the following solutions to the medium：8% NaHCO3 solution (filter-sterilized) 30.0ml 100 mM Fe NTA solution (see below) 50.0ml Adjust pH to 7.0 - 7.2100 mM Fe NTA solution:NaHCO3 1.64gNitrilotriacetic acid trisodium salt monohydrate 2.75gFeCl3·6H2O 2.7gFirstly dissolve NaHCO3 in 80 ml distilled water. Then add NTA and FeCl3·6H2O to the solution and bring volume to 100 ml with distilled water. Adjust pH to 7.0 with NaOH. Filter-sterilize and store under a N2 atmosphereTrace element solution：Nitrilotriacetic acid 12.8g FeCl3·6H2O 1.35g MnCl2·4H2O 0.1g CoCl2·6H2O 0.024g CaCl2·2H2O 0.1g ZnCl2 0.1g CuCl2·2H2O 0.025g H3BO3 0.01g NaCl 1.0g NiCl2·6H2O 0.12g Na2SeO3·5H2O 0.026g Distilled water 1.0L Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH. |
| 参考文献 | 参考文献内容 |
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