Description
产品说明
| 所属分类 | 微生物 > 模式菌株 |
|---|---|
| 品牌 / 来源 | CS-BIC | 中科生信 |
| 保存温度 | 冷藏4-10℃ |
| 培养温度 | 30℃ |
| 提供方式 | 冻干物 |
| 来源历史 | 咨询客服 |
| 模式菌株 | 非模式菌株 |
| 生物危害程度 | 四类 |
| 培养条件 | 0989KH2PO4 0.3gK2HPO4 0.3g(NH4)2SO4 0.3gNaCl 0.6gMgSO4·7H2O 0.13gFeSO4·7H2O 2.0mgCaCl2·2H2O 8.0mgYeast extract (Difco) 2.0gTrypticase peptone (BBL) 2.0gSodium acetate 2.5gSodium formate 2.5gTrace minerals (见培养基0893) 10.0mlTrace vitamins (见培养基0901) 10.0mlNaHCO3 5.0gNa2S·9H2O 0.5gL-Cysteine·HCl·H2O 0.5gResazurin 1.0mgDistilled water 1.0LMix ingredients except NaHCO3 L-Cysteine·HCl·H2O and Na2S·9H2O and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g. 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine·HCl and Na2S·9H2O as 5% solutions respectively under a N2 atmosphere. Prior to inoculation aseptically and anaerobically add the cysteine·HCl and Na2S·9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20). |
| 参考文献 | 参考文献内容 |
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