Description
产品说明
| 所属分类 | 微生物 > 模式菌株 |
|---|---|
| 品牌 / 来源 | CS-BIC | 中科生信 |
| 保存温度 | 冷藏4-10℃ |
| 参考用途 | 模式菌株 |
| 培养温度 | 60℃ |
| 提供方式 | 冻干物 |
| 来源历史 | 咨询客服 |
| 模式菌株 | 模式菌株 |
| 生物危害程度 | 四类 |
| 其它保藏中心编号 | =JCM 17260 |
| 培养条件 | THERMOSYNTROPHA L-60 MEDIUM 0977 THERMOSYNTROPHA L-60 MEDIUMSolution A:KH2PO4 0.54g Na2HPO4 1.33g Yeast extract (BD-Difco) 3.0g Tryptone (BD-Difco) 0.5g Bacto peptone (BD-Difco) 1.5g Resazurin 1.0mg Distilled water 760.0ml Solution B:NH4Cl 0.3g NaCl 0.3g CaCl2·2H2O 0.01g MgCl2·6H2O 0.1g Trace element solution (见培养基0901） 1.0ml Distilled water 100.0ml Solution C:Crotonic acid 1.72g Distilled water 100.0ml Adjust pH to 7.0Autoclave Solutions A B and C separately. After cooling aseptically and anaerobically combine the three solutions under a stream of N2-CO2 (80:20 v/v) then add the following solutions (filter-sterilized):Trace vitamins见培养基0901 10.0ml 8% NaHCO3 solution 30.0ml Prior to inoculation reduce the medium with 0.025% L-cysteine·HCl·H2O and 0.025% Na2S·9H2O (use 5% solutions autoclaved and stored under a N2 atmosphere). |
| 参考文献 | 参考文献内容 |
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