Description
产品说明
| 所属分类 | 微生物 > 模式菌株 |
|---|---|
| 品牌 / 来源 | CS-BIC | 中科生信 |
| 保存温度 | 4-10度 |
| 参考用途 | 模式菌株 |
| 培养温度 | 见说明书 |
| 提供方式 | 冻干粉 |
| 来源历史 | 咨询客服 |
| 模式菌株 | 非模式菌株 |
| 需氧类型 | 见说明书 |
| 生物危害程度 | 四类 |
| 其它保藏中心编号 | DSM467=ATCC11170 = NCIB8255 |
| 培养条件 | 红螺菌培养基 酵母提取物 0.30 g 乙醇 0.50 ml 琥珀酸钠 1.00 g 乙酸铵 0.50 g 柠檬酸铁(0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g 维生素B12 组份(10 mg in 100 ml H2O) 0.40 ml 微量元素组份 SL-6 (如下) 1.00 ml 蒸馏水 1050.00 ml pH 6.8. Boil the medium under a stream of nitrogen gas for few minutes and distribute 45 ml medium into 50 ml screw-capped bottles (already flushed with nitrogen gas). Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. For brown and other oxygen sensitive Rhodospirillaceae add 300 mg of L-cysteine (0.03% end concentration) to the boiling medium and readjust the pH to 6.8 or to the prepared medium in bottles inject neutralized sulfide solution (0.005 to 0.01% end concentration). The medium has been modified according to reference 3365. ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g 蒸馏水 1000.00 ml |
| 参考文献 | 参考文献内容 |
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