深红红螺菌Rhodospirillumrubrum

产品介绍
培养条件
红螺菌培养基
酵母提取物 0.30 g
乙醇 0.50 ml
琥珀酸钠 1.00 g
乙酸铵 0.50 g
柠檬酸铁(0.1% in H2O) 5.00 ml
KH2PO4 0.50 g
MgSO4x 7 H2O 0.40 g
NaCl 0.40 g
NH4Cl 0.40 g
CaCl2 x 2 H2O 0.05 g
维生素B12 组份(10 mg in 100 ml H2O) 0.40 ml
微量元素组份 SL-6 (如下) 1.00 ml
蒸馏水 1050.00 ml
pH 6.8.
Boil the medium under a stream of nitrogen gas for few minutes and distribute 45 ml medium into 50 ml screw-capped bottles (already flushed with nitrogen gas). Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples.
Incubate in the light using a tungsten lamp. For brown and other oxygen sensitive Rhodospirillaceae add 300 mg of L-cysteine (0.03% end concentration) to the boiling medium and readjust the pH to 6.8 or to the prepared medium in bottles inject neutralized sulfide solution (0.005 to 0.01% end concentration). The medium has been modified according to reference 3365.
ZnSO4 x 7 H2O 0.10 g
MnCl2 x 4 H2O 0.03 g
H3BO3 0.30 g
CoCl2 x 6 H2O 0.20 g
CuCl2 x 2 H2O 0.01 g
NiCl2 x 6 H2O 0.02 g
Na2MoO4 x 2 H2O 0.03 g
蒸馏水 1000.00 ml
参考文献
参考文献内容